FoxO targets in Tsc1-deficient cells under normal food and nutrient restriction food

Exploring downstream regulation by the transcription factor FoxO is necessary to understand its function as a tumor suppressor or promoter in different biological contexts. Here, we employed RNA-seq profiling on single clones isolated using laser capture microdissection from Drosophila larval eye imaginal discs to identify FoxO targets that restrict the proliferation of Tsc1-deficient cells under nutrient restriction (NR). Four single clones each were microdissected for isogenic control and Tsc1 mutant cells, with or without foxo knockdown, from larvae raised on normal food and NR. 31 cDNA libraries with sufficient material were sequenced. Differential gene expression analysis was performed on 29 samples that passed quality control.