Simul-seq Method. Multiple organisms

Paired DNA and RNA profiling is increasingly employed in genomics research to uncover molecular mechanisms of disease and to explore personal genotype and phenotype correlations. Here, we introduce a novel simultaneous DNA and RNA sequencing approach (Simul-seq) that enables comprehensive genomic and transcriptomic profiles from small quantities of cells or tissues. Using a variety of metrics, we demonstrate that Simul-seq produces genome-wide DNA and RNA sequencing data of high quality.