Transcriptional profiling of neurons in the cerebral cortex of a Kcnip2-TRAP (NIDA115) mouse line

TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in pyramidal neurons in the cerebral cortex of adult Kcnip2-EGFP/Rpl10a (NIDA115) mice. TRAP-Seq of neurons in the cerebral cortex of adult Kcnip2-EGFP/Rpl10a (NIDA115) mice翻译核糖体亲和纯化（Translating Ribosome Affinity Purification，TRAP）翻译谱分析技术是一种可使研究者对小鼠脑组织等复杂组织中的特定细胞类型进行遗传特征鉴定的方法。本研究采用该技术，从成年Kcnip2-EGFP/Rpl10a（NIDA115）小鼠大脑皮层的锥体神经元内的活跃翻译转录本中获取了RNA测序（RNA Sequencing，RNA-Seq）数据。针对成年Kcnip2-EGFP/Rpl10a（NIDA115）小鼠大脑皮层神经元的TRAP测序（TRAP-Seq）