single cell RNA sequecing of Gene epxressions and TCR clones in late-onset EAE

Aged patients with multiple sclerosis (MS) have more progressive and severe symptoms, even though aged people have increased frequency of peripheral Treg (pTreg) cells which inhibit this disease. Our goal is to how aged pTreg cells with changes in their frequency and functionality contribute to increased severity of late-onset MS using mouse model experimental autoimmune encephalomyelitis (EAE). For this purpose, we reported the Treg cell plasticities and T cell clonalities in the CNS infiltrated T cells in the late-onset EAE mouse model. single cell RNA-seq was performed on the the analysis of CNS infiltrated T cells in young and aged mouse.Treg plasticity was deterimined by the expression levels of il17a and ifng mRNA in Foxp3+ CD4 T cells. Clonality was deterimined by the frequency of clones in the whole TCR repertoire.