Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Toe1 konckout Transcriptomes in undifferentiated C17.2 cells. Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Toe1 konckout Transcriptomes in undifferentiated C17.2 cells

We extracted RNA from KO-NC and KO-Toe1 undifferentiated C17.2 cells for high-throughput transcriptomic sequencing.In undifferentiated C17.2 cells, Toe1 primarily influenced key biological processes such as calcium ion binding, the extracellular matrix (ECM), and alpha-amino acid catabolism Overall design: mRNA profiles of KO-NC and KO-Toe1 were generated by deep sequencing using Illumina GAIIx.