Kinetics of inhibition of FXa (0.15 µM) and thrombin (0.03 NIH U/ml) by Nk-PLA2α and Nk-PLA2β at 37°C, pH 7.4, respectively.
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The kinetic parameters (Km, Vmax, and Kcat) values were determined from Michaelis-Menten plot as described in the text. The chromogenic substrates (0.2 mM) used for the amidolytic activity assay of thrombin and FXa were T1637 and F3301, respectively. The values are mean of triplicate determinations and SD was found within 10% of the mean value.
创建时间：
2015-12-02
