Formalin-fixed paraffin embedded colorectal cancer and healthy colonic tissue sample collection 2

Formalin-fixed paraffin embedded (FFPE) tissues from colorectal cancer patients (n=14) and healthy controls (n=13) were obtained from pathology archives for 16S rRNA amplicon sequencing. The FFPE tissue collection includes n=11 colorectal cancer (CRC), n=14 normal adjacent (ADJ) and n=13 healthy control tissues. Controls included mock controls (n=2), PCR negatives (n=2) and paired empty paraffin samples (n=21) which were concurrently processed with the tissues. Additional samples consisted of DNA extraction negatives including blank (n=3) and water (n=3) controls.