RNAPII ChIP-Sequencing of A.thaliana tfiis mutant lines under heat stress conditions. RNAPII ChIP-Sequencing of A.thaliana tfiis mutant lines under heat stress conditions

RNAPII ChIP Sequencing in Col-0 and tfiis mutant lines to elucidate the role of TFIIS during RNAPII mediated transcription Overall design: Comparative ChIP-Sequencing using antibodies directed against S2P-modified (Ser2-phosphorylated heptapeptides of the carboxy-terminal domain of NRPB1) RNAPII (abcam, ab5095) in Col-0 and tfiis-1 under control conditions and heat shock conditions in biological triplicates