Supplemental Information for Structural and functional insights into acidophilic helicases as DNA-unwinding motors

This dataset contains the supplementary materials for the manuscript entitled “Structural and functional insights into acidophilic helicases as DNA-unwinding motors,” submitted to Applied and Environmental Microbiology (manuscript ID: AEM01670-25).The supplementary file includes additional figures (S1–S12) and tables (S1–S2) that provide supporting experimental data, cryo-EM analyses, protein purification and mutagenesis results, structural comparisons, and fluorescence-based assays. These materials complement the main text and offer detailed insights into the biochemical and structural characterization of the acidophilic helicase LfDda and its mutants.