Quantitative proteomics and phosphoproteomics reveal that PIM3 modulates cell migration via regulating Rho GTPase signaling

Stable cell line was used for this study, so there are minimum variations between samples. For the lable free based quantitative analysis of the whole proteome, three biological replicates for each condition were investigated. For the phosphoproteomic analysis, three biological replicates and two technical replicates were analyzed, resulting in twelve data for quantification. Statistical analysis was performed using student’s t-test