Hari Shroff, Catherine G Galbraith, James A. Galbraith, Helen White, Jennifer Gillette, Scott Olenych, Michael W. Davidson, Eric Betzig (2011) CIL:38602, Homo sapiens, fibroblast. CIL. Dataset
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This photoactivation localization microscopy (PALM) image of tdEos-paxillin (green) and PsCFP20-zyxin (red) demonstrates that these two focal adhesion proteins are have very little over-lap when visualized with super-resolution. In contrast, when the same field is visualized with total internal reflection (TIRF) microscopy, the two proteins appear almost completely colocalized CIL 38604. Note the differential interference contrast (DIC) image corresponding to the same image field is also available as CIL 38603. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 4 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
本图示为 tdEos-paxillin(绿色)和 PsCFP20-zyxin(红色)的图像,采用照片激活定位显微镜(PALM)技术获得。该图像表明,这两种焦点粘附蛋白在超分辨率成像下几乎无重叠。相对而言,当同一视野采用全内反射(TIRF)显微镜进行观察时,这两种蛋白几乎完全共定位。附图 CIL 38604 对应于该图像场差干涉对比(DIC)图像。图像刻度单位为 2 微米。该图像由 Catherine 和 James Galbraith 提供,并与 PNAS U S A 2007 年 12 月 18 日第 104 卷第 51 期的第 20308-13 页上的图 4 相对应。
提供机构:
CIL



