Transcriptome Analysis with Human Vascular smooth muscle cells under 2D and 3D cell culture conditions

The current objectives are to use of transcriptome sequencing analysis technology comprehensively understand the potential molecular mechanisms between vascular smooth muscle cells grown in 2D environments (2D-VSMCs) and vascular smooth muscle cells grown in 3D PGA environments (3D-VSMCs).These findings help in gaining a better grasp of the situation of how VSMCs survive on PGA scaffolds and have guiding significance for TEBV culture. Human vascular smooth muscle cells were cultured in 2D and PGA based 3D culture for 4 days. Total RNA was extracted using Trizol. RNA-SEQ was carried out to profile the gene expression in both culture conditions.