Microscopy analysis of A549 and H460 cells' mitochondria after exposure to TXT2 and TXT4
收藏Most Wiedzy Open Research Data Catalog2026-04-17 收录
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The data set contains live-cell imaging of A549 and H460 cells' mitochondria after exposure to TXT2 and TXT4. Cells were exposed for 6 h to the tested compounds at their IC90 concentration or to DMSO. Then, mitochondrial labelling was performed by incubating the cells with a MitoTracker Green FM probe (Thermo Fisher Scientific) and Hoechst 33342 (Thermo Fisher Scientific). Images were acquired with an LSM 800 inverted laser scanning confocal microscope (Carl Zeiss, Jena, German), equipped with an Airyscan detector for high-resolution confocal scanning using a ×63 1.4 NA Plan Apochromat objective (Carl Zeiss). Cells were incubated in an incubation chamber at 37°C with 5% CO2.
提供机构：
Natalia Maciejewska
