Bacterial enrichment prior to third-generation metagenomic sequencing improves detection of BRD pathogens and genetic determinants of antimicrobial resistance in feedlot cattle

Using nonselective bacterial enrichment improves the detection of pathogens associated with Bovine Respiratory Disease. Nanopore sequencing was used to characterize the taxonomic composition of samples identify determinants of antimicrobial resistance traits present in the microbiome.