EdU Optimization - Optimized Procedure Example
收藏NIAID Data Ecosystem2026-03-13 收录
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https://figshare.com/articles/dataset/EdU_Optimization_-_Optimized_Procedure_Example/20341446
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资源简介：
Flow cytometry data on mouse fecal bacteria incubated for three hours with (or without) 20 uM 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue, in a 1:1 ratio of reduced Brain Heart Infusion (rBHI) broth and fecal slurry (FS), to capture the actively replicating bacteria of healthy mice.
The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria.
In the file names:
w = with
wo = without
AF = Alexa Fluorphore 647
创建时间：
2022-07-20
