Antibodies were rabbit anti-HEC1 (green) antibody (Abcam) used at 1:1,000. and sheep anti-MCAK (red) (Andrews et al., 2004) used at 1 μg/ml, and DNA (blue). Fluorescently labeled secondary antibodies
A coumarin-derived complex, Hg2L2, was reported as a highly sensitive and selective probe for the detection of mercapto biomolecules in aqueous solution. The addition of Cys to a 99% aqueous solution
The growth cone was defined as the distal 10 microns of the axon shaft. If a growth cone exhibited lamellipodia then the distal most extent of the lamellipodium was taken as the distal most point on t