Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes

In order to better understand the biological roles of critical SARS-CoV-2 proteins, we determined and compared the host transcriptomic responses of the HL-CZ human promonocytic cell line upon transfection with key viral genes encoding the spike S1 subunit, S2 subunit, nucleocapsid (NP) protein, NSP15 (endoribonuclease), and NSP16 (2'-O-ribose-methyltransferase). Overall design: mRNA profiles of HL-CZ cells transfected with pCMV3 and pCMV3-C-FLAG as control vs. pCMV3-S1, pCMV3-S2, pCMV3-NP-FLAG, pCMV3-EN (NSP15), and pCMV3-ME (NSP16)