Transformation of NIH3T3 cells by the forced expression of wild-type YAP or its stabilized form. Mus musculus

YAP is known as a proto-oncogene with which the aberrant expression causes numerous types of cancer. To elucidate the molecular mechanisms underlying the acquisition of cancerous traits of normal cells by YAP overexpression, the wild-type YAP or its stabilized form was overexpressed in NIH3T3 cells. We found that the forced expression of either wild-type YAP or its derivative alone was sufficient for transforming NIH3T3 cells and we then analyzed the global expression profiles of the control NIH3T3 cells or those overexpressing wild-type or stabilized YAP. Overall design: The wild-type mouse Yap or the degradation resistant Yap in which serine at 112 was mutated by alanine was expressed in NIH3T3 cells.