Raw datasets_manuscript: Lysogenic control of bacterial morphology and fitness by Spbetavirus phi3T
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Raw data used to create the main and supplementary figures for the manuscript entitled Lysogenic control of bacterial morphology and fitness by Spbetavirus phi3T, by Floccari et al.Datasets supporting the various figures are divided into separate sheets, with each sheet titled according to the corresponding figure and panel. Main Dataset: Data source_Floccari et al This file contains data supporting the main figures, specifically: Figure 1g_f: Measurements of cell length and width taken manually using ImageJ software, listed in columns. Figure 3f: Measurements of spore length and width taken manually using ImageJ software, listed in columns. Figure 4a_b: OD readings (measured using a Biotek Synergy H1 plate reader) used to generate growth curves, along with raw colony counts for CFU/mL calculations. Figure 4d: OD, GFP, and mKate readings (from the Biotek Synergy H1 plate reader) used to generate competition assay graphs comparing WT and phi3T lysogen strains. Figure 5: OD readings (measured using the Biotek Synergy H1 plate reader) used to determine the minimal inhibitory concentration (MIC) of antibiotics. Figure 7d: OD, GFP, and mKate readings (from the Biotek Synergy H1 plate reader) used to create competition assay graphs comparing WT and double lysogen (DL) strains. Supplementary Dataset: Supplementary_Data source_Floccari et al This file contains data supporting supplementary figures, specifically: Supplementary Figures a_b: raw colony counts for sporulation frequency calculations. Supplementary Figures d_e: Fluorescence intensity measurements of sporulating cells taken in ImageJ, along with manual measurements of spore length and width using ImageJ, listed in columns. Supplementary Figures 6_7: OD readings (from the Biotek Synergy H1 plate reader) used to generate growth curves. Supplementary Figure 9: OD, GFP, and mKate readings (from the Biotek Synergy H1 plate reader) used to generate competition assay graphs comparing WT and SPβ lysogen strains. Supplementary Figure 10a: OD readings (from the Biotek Synergy H1 plate reader) used to determine the MIC of ampicillin. Supplementary Figure 10b: OD readings (from the Biotek Synergy H1 plate reader) used to determine the MIC of D-cycloserine. Supplementary Figure 11: OD readings (from the Biotek Synergy H1 plate reader) used to determine the MIC of ampicillin. Supplementary Figure 12: OD readings (from the Biotek Synergy H1 plate reader) used to determine the MIC of spectinomycin. Supplementary Figure 15: OD readings (from the Biotek Synergy H1 plate reader) used to generate growth curves.



