Airyscan confocal time-lapse data of cellulose synthase in developing protoxylem vessels of the Arabidopsis root.

These datasets are continuous time lapse of protoxylem vessels in roots of Arabidopsis thaliana. The aim is to study movement of cellulose synthase complexes (CSCs) during secondary wall formation.

Timecourse 1 is a three channel movie showing CFP-Microtubules (CFP:MBD), YFP-CSCs (YFP:CESA7) and mCherry-Actin (mCherry:fABD2). Frame interval is 6 s.
Timecourses 2, 3, 4 and 5 are single channel movies showing YFP-CSCs. Frame interval is 0.57s (timecourse 2) or 0.69s (timecourses 3, 4, 5).

All data before Airyscan software processing, containing signal from each of the sub-airy detectors, is provided in the .zip archives ("Airyscan_raw"). Extracted files are Zeiss .czi format and can be opened in Zeiss Zen confocal software.
Airyscan processed data is provided as .czi files ("Airyscan processed") and can be viewed in Zeiss ZEN software or ImageJ/Fiji BioFormats import tool. Multipage .tif files (see "steps to reproduce section") can be open natively by ImageJ/Fiji software.

For viewing fast dynamic processes the airyscan datasets are viewed without further processing. For viewing slower process (e.g. CSCs moving below the emerging secondary cell wall thickenings), the fast time-lapse data (timecourses2-5) are subjected to a running average (ImageJ/Fiji plugins "walking average" or "running Z projector") of x6.