RNA-seq after siRNA targeting DDX24 applied to iHUVECs cell lines

DDX24 Mutations Associated with Vascular Malformations To detect the vascular-related functions of the causative gene, two short interfering (si)RNAs targeting DDX24 were designed by GenScript bioinformatics tools (https://www.genscript.com/tools/sirna-target-finder) and transfected into immortal human umbilical vein endothelial cells (iHUVECs). A vehicle comprising scrambled siRNA (RIBOBIO) was used as a control. The knockdown efficiency was determined by real-time quantitative PCR and western blotting. RNA sequencing analysis was performed using the siRNA-targeted HUVEC cells to evaluate the effects of the causative gene knockdown on gene expression. Genes involved in cell migration were validated by real-time quantitative PCR.