Comparative analysis of the different Streptomyces bingchenggensis strains by RNA-seq

Streptomyces bingchenggensis is a soil bacterium that produces milbemycins. Milbemycins and their derivatives are valuable biopesticides in the agricultural field. Owing to their advantages such as high efficiency and safety for human and animal,it was urgent to construct high-yield strain to ensure low production cost. To obtain genes closely correlated with milbemycin production, we have compared the whole genome microarray expression profiling of two strains (the parent one strain and high-yielding strain). In Streptomyces bingchenggensis, there are abundant exporters, which are responsible for transporting various substrates. In the result, some drug exporters were chosen to enhance production of milbemycin . Overall design: 6 time points were designed at 18,48,72,96,144,and 192 hours in two strains (wild type and sbbA deletion mutant). The samples were collected at each time point named W1,W2,W3,W4,W5,W6, S1,S2,S3,S4,S5,and S6.