Transcriptome sequencing in Hearing loss

In this study we generated early otic lineage cells following the protocol of Matsuoka et al (PMID: 28186679). RNA extraction and purification were performed using TRIzol (Invitrogen) and RNAeasy Minikit (Qiagen), respectively. We sequenced the transcriptomes of iPSCs and otic progenitor cells derived from a patient (Clone) with homozygous deletion of Chr8:96,582,049-96,803,788 (hg19). We used ASE9023 (Control 1) and CD2 (Control 2) as control cells. We performed RNA-Seq on two clones (Clone 1 and 2) of the patient and CD2 and ASE9023 as control cells in technical duplicates (a and b) on days 3, 6 and 11.