Characterization of protein in the pistil after the compatible and incompatible pollination using

Analyze the different proteins in the pistil after the compatible and incompatible pollination of peony by iTRAQ. The number of identified proteins was 2900, 383 proteins of which were up-regulated expression, and 302 proteins were down-regulated. Those proteins mainly attended the metabolic pathways, biosynthesis of secondary metabolites, ribosome, protein processing in endoplasmic reticulum, spliceosome, RNA transport, glycolysis/gluconeogenesis etc., showed that the proteins and energy metabolism were active. Among them, the RNA degradation, calcium signaling pathway, MAPK signaling pathway, and phosphatidylinositol signaling system were involved in the cross-incompatibility between herbaceous peony and tree peony. Those showed that the energy synthesis of the growing pollen tubes and the polaized growth of pollen tubes were inhibited. Among Enolase, DnaK(HSP70), CALM were down regulating expression, and ANT was up regulating expression.