Comprehensive Map and Functional Annotation of Human Pituitary and Thyroid Proteome

Knowledge about human tissue
proteome will provide insights into health organ physiology. To construct
a comprehensive data set of human pituitary and thyroid proteins,
post-mortem pituitaries and thyroids from 10 normal individuals were
used. The pooled samples were prepared using two methods. One part
of the sample was processed using 14 high-abundance proteins immunoaffinity
column. The other part was directly subjected to digestion. Finally,
a total of 7596 proteins in pituitary and 5602 proteins in thyroid
with high confidence were identified, with 6623 and 4368 quantified,
respectively. A total of 5781 of pituitary and 3178 of thyroid proteins
have not been previously reported in the normal pituitary and thyroid
proteome. Comparison of pituitary and thyroid proteome indicated that
thyroid prefers to be involved in nerve system regeneration and metabolic
regulation, while pituitary mainly performs functions of signal transduction
and cancer modulation. Our results, for the first time, comprehensively
profiled and functionally annotated the largest high-confidence data
set of proteome of two important endocrine glands, pituitary and thyroid,
which is important for further studies on biomarker identification
and molecular mechanisms of pituitary and thyroid disorders. The mapping
results can be freely downloaded at http://www.urimarker.com/pituitary/ and http://www.urimarker.com/thyroid/. The raw data are available via ProteomeXchange with identifier
PXD006471.