Smarcb1 plays a role in fidelity of pluripotency

Functions of SMARCB1, one of the core subunits of BAF complexes, is not fully characterized in embryonic stem (ES) cells due to inability to establish ES cells from Smarcb1-deficient embryos. We conducted RNA-seq and ATAC-seq studies in combination with the CRISPR/Cas9-mediated genetic depletion to solve the problem. Consequently, we found that SMARCB1 plays a role in maintenance of pluripotency and differentiation fidelity of ES cells, which is potentially through regulation of chromatin landscapes.