MAb reactivities for DENV-2 virus-like particle (VLP) mutants1.

1Reactivities levels of MAbs of varying cross-reactivity (CR) selected from different flaviviruses for wildtype (WT) and mutant VLP antigens.2Rabbit anti-DENV-2 VLP-immunized hyper-immune sera used as detector for antigens captured with MHIAF.3Antibody Cross-reactivity (CR): Rabbit anti-DENV-2 and murine hyper-immune ascitic fluid (MHIAF) are polyclonal; ‘group’ CR antibodies recognize viruses of the four major pathogenic flavivirus serocomplexes; ‘sub-group’ CR MAbs recognize all or some members of two or more different flavivirus serocomplexes (e.g., MAbs 20, 5-1 and 5-2 recognize DENV-2 and JEV, JEV and DENV-2 and JEV, DENV-1 and DENV-2 respectively); ‘comp.’ and ‘sub-comp.’ CR MAbs recognize all four DENV complex viruses or a subset thereof respectively, and type MAbs recognize only DENV-2.4Virus the MAb was raised against; D1 = dengue virus serotype-1 (DENV-1), D2 = DENV-2, D3 = DENV-3, D4 = DENV-4, SLEV = St. Louis encephalitis virus, MVEV = Murray Valley encephalitis virus, WNV = West Nile virus, and JEV = Japanese encephalitis virus.5Percent secretion of mutant VLP constructs relative to WT. All values are the average of three independent experiments.6MAb reactivities for WT DENV-2 VLP are presented as inverse log10 Ag-capture ELISA endpoint values and mutant VLPs as the percent remaining reactivity compared to WT. Emboldened values represent reactivity reductions greater than 90% relative to WT.7nd denotes not determined.