IC₅₀ values for autologous plasma/mAbs with R880F wild-type and mutant Envs.

aLongitudinal wild-type and mutant Envs were assayed for neutralization sensitivity to autologous plasma and mAbs 19.3H-L1 and 19.3H-L3 in Tzm-bl cells. Average IC50 values for two independent experiments using duplicate wells are given in the second through fifth columns. bAutologous plasma samples assayed in the second column were contemporaneous with envelope isolation dates (e.g. 0-month plasma with 0-month Envs, 2-month plasma with 2-month Envs, etc.). cnd, not done. d19.3H-L1 and 19.3H-L3 were isolated from a 16-month cryopreserved R880F PBMC sample. eThese envelopes, though technically demonstrating IC50 values greater than 25 µg/ml, saw slight neutralization at the highest concentration of antibody tested. fThe four right-most columns detail which amino acids appear at the specified Env residues (295, 335, 338, and 341). N* denotes the introduction of a potential N-linked glycosylation site, while N** marks where such a site has been shifted downstream from a previously existing site in the Env sequence.