CD19-CAR T cells undergo exhaustion epigenetic programming in patients with acute lymphoblastic leukemia

Zebley et al. show that CD8+ CD19-CAR T cells undergo genome-wide DNA methylation changes during an anti-tumor response in patients with B-cell acute lymphoblastic leukemia (ALL). Post-infusion CAR T cell differentiation involves acquisition of DNA methylation programs associated with effector function, repression of memory potential, and transition toward exhaustion. 4 Pre-infusion samples, 13 GMP samples, 4 week1 after GMP samples, 5 week2 after GMP samples, 3 week3 after GMP samples, 1 week4 after GMP sample.