Suppression of inflammaging prolongs hematopoietic healthspan [dataset 2]

The impact of recombinant Thbs1 (rThbs1) on HSC activity is unknown. In this experiment, RNA Seq analysis was performed on BM HSCs derived from young (4 month-old) control mice that were cultured ex vivo for 11 days in the presence or absence of rThbs1 (500 ng/mL). Additionally, RNA Seq analysis of HSCs cultured with rThbs1 in the presence of its neutralizing antibody (Clone A4.1) or its isotype control was performed to identify the pathways that are altered in HSCs upon exposure to rThbs1. Overall design: Comparative gene expression profiling analysis of BM HSCs cultured in the presence of recombinant Thbs1 and its neutralizing antibody.