scRNA sequencing analysis of neutrophils and sphingolipid pathway during virus persistence

During lymphocytic choriomeningitis virus (LCMV) persistence, neutrophils were shown to be regulated, but the underlying mechanisms remain unclear. In this study, we have employed a scRNA analysis using bone marrow cells during LCMV infection. The experiment was conducted to define the possible role of sphingosine kinase 2 (SphK2) in neutrophil regulation upon LCMV infection. The findings may increase our understanding of viral mechanisms to regulate the host immunity and virus persistence. Overall design: WT and Sphk2-/- knockout C57BL/6 mice (n=3 mice/group) were infected with LCMV Cl 13. Bone marrows cells were collected from uninfected WT and LCMV Cl 13-infected WT and Sphk2–/– mice at 3 dpi and analyzed using scRNA-seq.