MCPIP3 competes with IGF2BP3 binding to common stem-loop structure in the 3’UTRs of METAP2 gene for suppressing lung cancer metastasis

This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human lung cancer cell A549, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and then identified by high resolution mass spectrometry.