Genomic distribution of H3K9me2 in wild-type vs. arle-14 mutant C. elegans embryos

We discovered arle-14/B0336.5 as a critical gene for H3K9 di-methylation. To test the hypothesis that ARLE-14 targets the H3K9 methyltransferase MET-2 to specific loci, we compared the distribution of H3K9me2 in wild-type and arle-14 mutants by ChIP-Seq. Our results reveal that H3K9me2 genomic distribution is similar in wild-type vs. mutants and ARLE-14 is not required for targeting MET-2. We used two biological replicates for each genotype (wild-type and arle-14 mutant). Input DNA was used as a control for each sample. arle-14 mutants have overall lower levels of H3K9me2. In this experiment, we collected 3 times more material from arle-14 mutants compared to wild-type to get enough material for sequencing. We note that the results are not quantitative in terms of H3K9me2 levels.