Dataset for: Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study

The study material in the GWAS panel with 282 advanced breeding lines of
bread wheat genotypes from IARI stress breeding program was selected to
map the genomic regions responsible for grain iron and Zinc content under
drought and heat stress treatments. Phenotypic data: The GWAS panel was
evaluated at IARI, New Delhi - DL (28.6550° N, 77.1888° E, MSL 228.61 m)
under Irrigated (IR), Restricted Irrigated (RI) and Late sown (LS)
treatment conditions with augmented RCBD design. Data was collected on
Grain Iron and Grain zinc content along with thousand-grain weight. Around
20 g of grain sample from each of 282 genotypes from the GWAS panel under
all three conditions were used for phenotyping GFeC and GZnC through
high-throughput Energy Dispersive X-ray Fluorescence (ED-XRF) machine
(model X-Supreme 8000; Oxford Instruments plc, Abingdon, United Kingdom)
calibrated with glass beads-based values. To record TGW, manual counting
of grains was followed and the weight of the grains was recorded in grams
with an electronic balance. Genotypic data: Genomic DNA of the GWAS panel
was extracted from the leaves of seedlings by Cetyl Trimethyl Ammonium
Bromide (CTAB) method. The panel was genotyped using Axiom Wheat Breeder’s
Genotyping Array (Affymetrix, Santa Clara, CA, United States) having 35,143
genome-wide SNPs. The monomorphic, markers with minor allele frequency
(MAF) of <5%, missing data of >20%, and heterozygote
frequency >25% were removed from the analysis. The remaining set of
10546 high-quality SNPs was used in GWAS analysis.