Transcriptomic analysis of tumor-infiltrating dendritic cells in mouse subcutaneous tumor models

CCR7+ tumor-infiltrating DCs (tDCs) transport tumor antigens from the tumor to the draining lymph node (dLN), and thus play a pivotal role in anti-tumor CD8+ T cell responses. However, the molecular mechanisms that underlie tDC activity remain poorly understood. To elucidate this problem, we investigated the transcriptomic properties of CCR7+ tDCs in mouse subcutaneous tumor models. Overall design: In the LLC and B16F10 subcutaneous tumor models, tumor-infiltrating lineage-negative (CD3, CD19, NK1.1, Ly-6G, Ter-119)- Ly-6C- MHC class II+ CCR7+ tDCs, lineage- CCR7- MHC class II+ cells and CD326- migratory DCs in the dLN were purified by cell sorting at day 10 or day 14 post-tumor inoculation. Then, we performed transcriptome analysis on these cells by 3' SAGE-seq.