Rad51-Dmc1 co-foci occur in pairs separated by 200–400 nm.

(A) Spread wild type diploid [WT (2N)] nucleus stained for Rad51 (green) and Dmc1 (red). (B,C,D) Spread spo11 hypomorphic tetraploid [spo11 hypo (4N)] nuclei with relatively low densities of Rad51-Dmc1 staining. Arrowheads indicate examples of paired co-foci in less densely stained areas, magnified in the insets. Scale bar = 1 μm, 400 nm for the inset. (E) Schematic of distance to nearest neighbor measurement for Rad51-to-Rad51 (left) and Rad51-to-Dmc1 (right). Solid arrow represents the distance to the reference focus’ nearest neighbor. Dashed arrows represent the longer distances to other foci. (F,G,H,I) Distribution of nearest neighbor measurements in wild type spo11 hypomorphic tetraploid. Experimental data (red) and matched random simulations (blue) shown for (F) Rad51→Rad51, (G) Dmc1→Dmc1, (H) Rad51→Dmc1, and (I) Dmc1→Rad51. Micrographs from cultures 2.5 hours after meiotic induction. Scoring of 195 focus-positive nuclei, of all staining densities, containing a total of 13,528 Rad51 foci and 13,230 Dmc1 foci are included in the histograms.