Regional epithelial cell diversity in the small intestine of pigs

Single cell suspensions enriched for epithelial cells were obtained from duodenum, jejunum, and ileum of a 7.5-week-old pig and subjected to single-cell RNA sequencing (scRNA-seq). scRNA-seq was performed to provide transcriptomic profiles of epithelial cells, with 695 cells annotated into 6 cell types. Deeper interrogation of data revealed previously undescribed cells in porcine intestine, and region-specific gene expression profiles within specific cell subsets. Data herein includes a .h5seurat files of the epithelial cell subsets analyzed. Files may be used to reconstruct different analyses and perform further data query. Scripts for original data analyses are found at https://github.com/USDA-FSEPRU/scRNAseqEpSI_Pilot. Raw data are available at GEO accession GSE208613. Data are available for online query at https://singlecell.broadinstitute.org/single_cell/study/SCP1936/regional-epithelial-cell-diversity-in-the-small-intestine-of-pigs.

Resources in this dataset:
Resource Title: .h5Seurat object - epithelial cells.
File Name: EpithelialCells.tar
Resource Description: Epithelial cells used for data analysis, available in .h5Seurat file format. Untar file before use.