Mutations in FMDV populations subjected to mutagenesis (FUG-200 combination).

aThe populations correspond to those described in Figure 2 [either WT or M296I subjected to 2, 5, 8 or 10 passages in absence or presence of R 5 mM] and then, in all cases, subjected to 5 passages in the presence of [FU(200 µg/ml)+GU (4 mM); FUG-200 combination]; +R means that passages were carried out in the presence of 5 mM ribavirin.bThe FMDV genomic region analyzed. In all cases, the region that was sequenced spanned the entire 2C-coding region; residue numbering is according to [74].cIt indicates whether the RT-PCR amplification of the genomic region given in the second column produced sufficient DNA for sequencing (+) or not (−). The primers used for the RT-PCR amplification, and the conditions for amplification are detailed in Materials and Methods.dMutations and deduced amino acid substitutions found. Two residues separated by a bar indicate a mixture of two nucleotides (and deduced amino acids) in the population, according to the sequencing band pattern. N.A. means not analyzed. Procedures for nucleotide sequencing and identification of FMDV genomic regions are described in Materials and Methods.