Y2H validation of the necdin and cystin interaction.
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+LgT and Lam+LgT were used as positive and negative controls, respectively for protein interaction. Necdin was identified from a cDNA library as a full-length cystin binding partner. LgT: large T-antigen; Lam: human lamin C. pGBKT7 is a bait vector containing the GAL4 DNA binding domain; pGADT7 and pACT2 are prey vectors containing the GAL4 activation domain. p53
创建时间：
2015-12-02
