Whole genome mapping of heterochromatin markers and their effects on transcription in migrating cells

Previously, H3K9me3, H3K27me3 and H4K20me1 increase levels in migrating cells were determined by immunostaining, a technique that is not able to localize the histone modifications to specific genomic loci. Here, we analyze for the first time the genomic distribution of H3K9me3, H3K27me3 and H4K20me1 in migrating cells in high resolution using chromatin immunoprecipitation following by sequencing (ChIP-seq). Associations between these histone modifications and cellular transcriptional profile is also measured by RNA-seq .