Catalysis of L-cysteine by IscS in the presence of IscU (dataset formatted for analysis by interferENZY)

Main description

This dataset depicts the catalysis of L-cysteine to sulfur by the desulfurase IscS in the presence of the scaffold protein IscU. This kinetic system was described in the PhD thesis "New tools for enzymatic characterization and inhibitor screening: post-Michaelis-Menten kinetic analysis" by Maria Filipa Pinto (https://hdl.handle.net/10216/126202), and this dataset was then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis.

Dataset organization

Line 1: Tab-separated initial concentrations of substrate L-cysteine in micromolar (µM) concentration

Line 2: Concentration of enzyme (1 µM)

Line 3: Units of time

Line 4: Units of concentration for substrate values and measurements

Line 5: Dataset name

Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1

Contact information:

Maria Filipa Pinto ([e-mail])
Pedro M. Martins ([e-mail])

i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: [phone]