Novel Approach to High-Throughput Identification and Characterization of Neoantigens

This study focuses on the development of novel approach for the screening, identification and characterization of neoantigen-specific T-cell receptors (TCRs). The developed method consists of collecting targeted RNA-Seq from a T-cell specific gene panel via 3' end Illumina sequencing combined to Oxford Nanopore based full-length TCR sequencing post T-cell activation. The data generated during this study demonstrates feasibility of the approach on a Jurkat control cell line as well as T-cells isolated from the blood of 3 independent healthy donors.]]>
In order to be eligible for participation in this study, subjects must meet the following criteria: capable of giving consent healthy subjects willing and able to provide blood]]>