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Data for: Defective kernel 66 encodes a GTPase essential for kernel development in maize

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DataONE2023-09-27 更新2024-06-08 收录
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Mitochondria is a semi-autonomous organelle that provides energy for cell activities through oxidative phosphorylation. In this study, we identified a defective kernel 66 (dek66)-mutant maize with defective kernels. We characterized the candidate gene DEK66, encoding a ribosomal assembly factor, located in mitochondria, and possessing GTPase activity (the GTPase belonged to ribosome biogenesis GTPase A family). In the dek66 mutant, abolishment of mitochondrial structure and function led to the accumulation of reactive oxygen species and promoted programmed cell death in endosperm cells. Furthermore, transcript levels of most of the key genes associated with nutrient storage, mitochondrial respiratory chain complex, and mitochondrial ribosomes in the dek66 mutant were significantly altered. Collectively, the results suggested that DEK66 is essential for the development of maize kernels via affecting mitochondrial function. This study provided a reference to understand the impact of mitoc..., , , # Data for: Defective kernel 66 encodes a GTPase essential for kernel development in maize Primary data in experimental procedures and results.This file is intended to provide key information for understanding and reuse of the dataset “Defective kernel 66 encodes a GTPase essential for kernel development in maize”. ## Description of the data and file structure Each figure plate corresponds to a sheet in the excel file. The content marked in bright yellow and bright blue in each datasheet represent the Figure number and line headers, respectively. The contents in parentheses in the line headers is the unit of each parameter. 1. Fig. 1G The statistical data of the length of per kernel of WT and dek66 2. Fig. 1H The statistical data of the width of per kernel of WT and dek66 3. Fig. 1I The statistical data of hundered-grain weight of WT and dek66 4. Fig.2D Quantification of zein, non-zein, and total protein contents in WT and dek66 mature endosperm repeat 1, repeat 2, repeat 3 tec...
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2023-11-29
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