RNA-sequencing for Bnt07G011994-overexpressed Arabidopsis

Bnt07G011994 is a ramie KNOX gene, and its overexpression caused a significant decrease in fiber number in transgenic Arabidopsis, indicating a negative role of this gene in modulating fiber growth. To obtain insights into the potential functional mechanism of Bnt07G011994, we performed RNA sequencing for wild and Bnt07G011994-overexpressed Arabidopsis. After comparing their transcriptomes, a total of 251 differentially expressed genes were identified. Wild and Bnt07G011994-overexpressed Arabidopsis were grown in a greenhouse at 22°C under a 15 h light/ 9 h dark cycle. When the stems reached to about 10 cm-length, stems of each individual were collected as a sample for RNA sequencing.