RNA-seq of neuronal SOX2-ablated murine suprachiasmatic nuclei against wild-type littermate controls

Mice lacking the stem/progenitor cell transcription factor, Sex determining region Y-box 2 (SOX2), in neurons of the suprachiasmatic nuclei display imprecise, poorly consolidated behavioral rhythms that do not entrain efficiently to environmental light cycles. RNA sequencing revealed that Sox2 deficiency alters the circadian transcriptome of the SCN, reducing the expression of many core clock genes and neuropeptide-receptor systems.在视交叉上核（suprachiasmatic nuclei, SCN）神经元中缺失干/祖细胞转录因子——性别决定区Y框蛋白2（SOX2）的小鼠，表现出节律不精确、巩固程度欠佳的行为节律，且无法高效地与环境光周期建立同步。通过RNA测序（RNA sequencing）分析发现，Sox2缺失会改变视交叉上核的昼夜节律转录组，下调大量核心时钟基因与神经肽受体系统的表达水平。