Analysis of hybrid F1 plants co-transgenic for both integrase and target sequence.

The results of the PCR analyses are summarized. The copy number of the target sequence was estimated by DNA gel blot analysis.Independent co-transgenic hybrid plants carrying both the HW511 and either the ICH13130 or the ICH14313 loci, or a recombinant derivate were included in the analysis. Descendants that lost the target locus or the integrase locus or both due to segregation in meiosis of T0 plants are not listed in the table.“+”/“−“ indicates the presence/absence of recombination.*1 Primary transformants (T0) that were hemizygous for the target locus were crossed with the integrase lines.*2 PCR using primers RecFw and RecRev resulted in the production of a 400 bp fragment containing attR (Fig. 1 and 2). The number of individuals that contained a recombination event and a non-recombinant locus (which was identified by PCR-amplification of GFP with the primers gfpFw and gfpRev and/or by the amplification of a 1.5 kb fragment using primers RecFw and RecRev) is indicated in brackets.*3 PCR using primers RecFw and RecRev resulted in the production of a 1.5 kb fragment containing GFP (Fig. 1 and 2).