Effect of Pumpkin Seed Peptide on Improving Sports Fatigue

In this study, a fatigue mouse model was developed to investigate the effects of different doses of pumpkin seed peptide on fatigue in mice. Forty-five mice were randomly assigned to five groups: normal control group, model group, and three pumpkin seed peptide groups with low, medium, and high-doses. Firstly, the amino acid composition of the pumpkin seed peptide was analyzed. Secondly, the changes in swimming time, serum harmful substances, energy substances, oxidative stress, and inflammation levels in mice were examined following intervention with different doses of pumpkin seed peptide. The results revealed that the pumpkin seed peptide contained 18 amino acids, including Glu, Leu, and Pro. Animal experiments demonstrated that compared to the model group, swimming time, muscle glycogen, and hepatic glycogen levels were significantly increased (PPPPPα, and IL-1β) were significantly reduced (PPP<0.05) with pro-inflammatory cytokines and serum markers (lactate dehydrogenase and urea nitrogen). In conclusion, pumpkin seed peptide demonstrates anti-fatigue properties, with high-dose administration exerted the most pronounced effects. This study provides a theoretical foundation for the application of pumpkin seed peptide in fatigue management.