H3K27ac Hi-ChIP analysis in Jurkat and RPMI-8402 T-ALL cells

H3K27ac Hi-ChIP analysis was performed in Jurkat and RPMI-8402 stably expressing dCAR9-KRAB after treatment with DMSO (Control) or Doxycycline to induce sgRNA expression to block MYCN enhnacer region Cells were treated with DMSO or doxycycline for 48 hours. The cells were subsequently collected and crosslinked with formaldehyde for 10 min. Hi-ChIP was performed using an antibody against H3K27ac (Abcam: ab4729). Cells were first lysed and then incubated with antibody against H3K27ac histone mark. DNA was enriched by chromatin-immunoprecipitation (ChIP), tagmented with Tn5, subjected for library construction, and sequenced by the DNBseq platform.