Light-induced changes of thylakoid grana structure

This dataset encompasses comprehensive analyses of thylakoid membrane structural dynamics in response to light exposure. The study investigates temporal changes in thylakoid network organization, particularly focusing on grana structural modifications during dark-to-light transitions.File name: CLSM_numerical_data.csv – volumetric data and thickness of the thylakoid network from dark and light conditions of Arabidopsis and Ficus plants grown in greenhouse conditions. V denotes volume in µm3 units; A denotes area in µm2 units; T denotes thylakoid thickness in µm units; sign "-" denotes lack of the data.File name: TEM_numerical_data.csv – TEM structural parameters of grana thylakoids of Arabidopsis and Ficus from dark and light conditions. SRD denotes stacking repeat distance in nanometer units. LF denotes percentage (%) of lumen fraction in SRD change. GD denotes grana diameter, GH denotes grana height both in nanometers units; sign "-" denotes lack of the data.File name: immunoblot_densitometry.csv – densitometric analysis of immunodetection of Lhcb5, Lhcb4, Lhcb2, Lhcb2-P, D1, D1-P, Lhca1, AtpB, PsaB in thylakoid samples of Arabidopsis and Ficus from dark and light (30, 50, and 90 min) conditions. Column A – gel lane No; column B – sample description; columns C-AF – pixel intensity data.File name: immunoblots.zip – compressed 31 files containing 30 files in tiff or jpg format and 1 readme.txt file. The Images represent immunoblots of thylakoid proteins: Lhbc5, Lhcb4, Lhcb2, Lhcb2-P, D1, D1-P, Lhca1, AtpB, PsaB for Arabidopsis and Ficus plants from dark conditions (0 min) or exposed to light (30, 50, and 90 min). The sample loading from left to right: Arabidopsis 0 min, Ficus 0 min, Arabidopsis 30 min, Ficus 30 min, Arabidopsis 50 min, Ficus 50 min, Arabidopsis 90 min, Ficus 90 min.File name: 77_K_fluorescence_spectra.csv – raw data for chlorophyll emission spectra of Arabidopsis and Ficus thylakoids isolated from dark conditions.File name: P515_single_light_intensity_data.csv – raw data of P515 signal (recorded on Dual-PAM device with P515 module) at single actinic light intensity (500 µmol of photons m-2 s-1) for dark-adapted Arabidopsis and Ficus plants.File name: P515_light_curve_data.csv – raw data of P515 signal (recorded on Dual-PAM device with P515 module) at increasing actinic light intensities 15, 99, 309, 773, 1764, and 3963 µmol of photons m-2 s-1 for dark-adapted Arabidopsis and Ficus plants.