anchialine metagenome Raw sequence reads

Microbial ecology is an important and growing field that has revealed the importance of microbes in many ecosystems; however, some ecosystems have yet to receive much attention including the anchialine ecosystem (nearshore bodies of water with subsurface freshwater and seawater connections). Hawaiian anchialine habitats in the Cape Kinau region of Maui and the Kona region of Hawaii exhibit distinctive, laminated orange cyanobacterial-bacterial crusts, but little is known about their composition, the composition of microbial communities in non-crust containing sites lacking the cyanobacterial-bacterial crusts, or the degree to which environmental factors structure them. During the summer of 2010, we surveyed benthic and water column microbial communities from ten anchialine habitats on Oahu, Maui, and Hawaii using high-throughput amplicon sequencing of the V6 (prokaryotic-biased) and V9 (eukaryotic-biased) hypervariable regions of the SSU rRNA gene. A subset of six anchialine habitats were also sampled in the spring, summer, and winter of 2011 to allow elucidation of seasonal effects on the Hawaiian anchialine microbial community.

微生物生态学作为一门重要且日益发展的学科，揭示了微生物在众多生态系统中的关键作用；然而，某些生态系统，如咸淡水体生态系统（近岸水体，具有地下淡水和海水相连的特点），尚未得到充分的关注。夏威夷群岛的卡伊纳角地区毛伊岛和科纳地区的咸淡水体栖息地展现出独特的、层状橙色的蓝细菌-细菌皮壳，但对于其组成、不含蓝细菌-细菌皮壳的非皮壳位点微生物群落组成，以及环境因素对其结构的塑造程度，了解甚少。在2010年夏季，我们利用高通量扩增子测序技术，对夏威夷群岛欧胡岛、毛伊岛和夏威夷岛上的十个咸淡水体栖息地的底栖和水柱微生物群落进行了调查，测序区域包括SSU rRNA基因的V6（细菌偏向）和V9（真核生物偏向）高度可变区。此外，2011年的春季、夏季和冬季，我们还对六个咸淡水体栖息地进行了采样，以阐明季节性变化对夏威夷咸淡水体微生物群落的影响。